Fig. 2

Knockdown of Srg3 suppresses lung injury symptoms in septic rats. A Schematic diagram of injecting the constructed AAV9–shRNA vector targeting Srg3 into rats via the tail vein. B, C qRT–PCR and WB detection of Srg3 mRNA and protein expression levels in rat lung tissue. D H&E staining to detect pathological changes in rat lung tissue. E PAS staining to observe the proportion of glycogen in rat lung tissue. F Flow cytometry analysis of the number of various immune cells in rat BALF. G ELISA detection of inflammatory factors in rat BALF. H, I IHC staining to observe the staining intensity of iNOS and Arg1 in rat lung tissue. J EB staining to observe vascular permeability in rat lung tissue. K IHC staining to observe the staining intensity of Spc-1 in rat lung tissue. L TUNEL staining to detect the proportion of apoptotic cells in lung tissue. Each group contained six rats, with each point representing one rat. Significant differences were detected using one-way ANOVA and Tukey’s multiple comparison test, **P < 0.01, ***P < 0.001, ***P < 0.0001