Fig. 4

Srg3-regulated NF-κB signaling pathway and ferroptosis. A We performed KEGG enrichment analysis to identify signaling pathways associated with Srg3-regulated genes. B Protein expression levels of p65, phos-p65, Ikbkb, and phos-Ikbkb were measured in rat lung tissue. C, D Immunohistochemistry was used to determine the staining intensity of phos-p65 and phos-Ikbkb in rat lung tissue. E Immunofluorescence was used to determine the nuclear localization of phos-p65 in rat lung tissue. F Protein expression levels of p65, phos-p65, Ikbkb, and phos-Ikbkb were measured in BEAS-2B cells by WB. G Immunofluorescence was used to determine the nuclear localization of phos-p65 in BEAS-2B cells. H, I Immunohistochemistry was used to determine the staining intensity of Gpx4 and Cox-2 in rat lung tissue. J, K qRT–PCR and WB were used to measure mRNA and protein expression levels of Gpx4 and Cox-2 in BEAS-2B cells. Each experiment was repeated three times, and the data were presented in the form of mean plus or minus standard deviation. Each group contained six rats, with each point representing one rat. One-way ANOVA or two-way ANOVA was used for significance analysis between the data. After ANOVA, Tukey’s multiple comparison test was used for post hoc test. **P < 0.01, ***P < 0.001, ***P < 0.0001