Fig. 5

Phorbol esters and erastin treatment weakened shSrg3 function on septic lung injury. A Schematic diagram of injecting the constructed AAV9–shRNA vector targeting Srg3 into rats via the tail vein, then phorbol esters and erastin treatment. B H&E staining to detect pathological changes in rat lung tissue. C PAS staining to observe the proportion of glycogen in rat lung tissue. D Flow cytometry analysis of the number of various immune cells in rat BALF. E ELISA detection of inflammatory factors in rat BALF. F, G IHC staining to observe the staining intensity of iNOS and Arg1 in rat lung tissue. H EB staining to observe vascular permeability in rat lung tissue. I IHC staining to observe the staining intensity of Spc-1 in rat lung tissue. J TUNEL staining to detect the proportion of apoptotic cells in lung tissue. Each group contained six rats, with each point representing one rat. Significant differences were detected using one-way ANOVA and Tukey’s multiple comparison test, **P < 0.01, ***P < 0.001, ***P < 0.0001