Fig. 6

Irf7 transcriptionally regulates Srg3 and activates its transcriptional activity in sepsis-induced acute lung injury. A Prediction of transcription factors that can bind to the Srg3 promoter using hTFtarget and JASPAR websites. B Identification of the conserved binding sequence of Irf7. C Verification of the binding relationship between Irf7 and the Srg3 promoter using ChIP–qPCR experiments. D Construction of a luciferase reporter vector containing the Srg3 promoter sequence and transfection into HEK293T cells along with an overexpression vector of Irf7 to measure luciferase activity. E, F Measurement of Srg3 mRNA and protein expression levels in BEAS-2B cells after overexpression of Irf7. G, H Measurement of Irf7 mRNA and protein expression levels in rat lung tissue using qRT–PCR and WB, respectively. Each experiment was repeated three times, and the data were presented in the form of mean plus or minus standard deviation. Each group contained six rats, with each point representing one rat. One-way ANOVA or two-way ANOVA was used for significance analysis between the data. After ANOVA, Tukey’s multiple comparison test was used for post hoc test. **P < 0.01, ***P < 0.001, ***P < 0.0001