Fig. 7

The overexpression of Irf7 weakens the beneficial effect of Srg3 knockdown on improving sepsis-induced acute lung injury. A qRT–PCR analysis was performed to detect the mRNA expression levels of Srg3 and Irf7 in BEAS-2B cells. B WB analysis was performed to detect the protein level of Srg3, Irf7, phos-p65, and phos-Ikbkb. C Immunofluorescence was used to determine the nuclear localization of phos-p65 in BEAS-2B cells. D, E CCK-8 and EdU staining were used to analyze the growth activity of BEAS-2B cells. F DAPI staining was used to analyze the nuclear morphology of BEAS-2B cells. G Flow cytometry was used to detect the proportion of CD86 and CD206 in THP-1 cells after coculture. H Immunofluorescence staining was used to detect the fluorescence intensity of iNOS and Arg1 in THP-1 cells after coculture. Each experiment was repeated three times, and the data were presented in the form of mean plus or minus standard deviation. One-way ANOVA or two-way ANOVA was used for significance analysis between the data. After ANOVA, Tukey’s multiple comparison test was used for post hoc test. **P < 0.01, ***P < 0.001, ***P < 0.0001