Fig. 6

A conserved ZFX/WNT3 axis modulates the growth and imatinib response mesylate of BCR/ABL⁺ cells. A The similarity analysis of ZFX proteins of various species is shown. B The promoter regions of WNT3 of various species were subjected to alignment analysis. A key ZFX binding site (GGGCCGGGCGG) is highlighted in the red box. C The relative expression of Wnt3 in BaF3–BCR/ABL cells upon Zfx silencing was analyzed by RT–qPCR. D The relative expression of Wnt3 in BaF3–BCR/ABL cells upon Zfx overexpression was assessed by RT–qPCR. E–G WNT3 and the empty control (Ctrl) were delivered into the control (Scramble) and ZFX-silenced K562 cells, the growth (n = 4), CFC production (n = 4), and imatinib mesylate (IM) response (n = 3) of variously transduced cells were measured. H–J WNT3 and the empty control (Ctrl) were delivered into the control (scramble) and Zfx-silenced BaF3–BCR/ABL cells, the growth, CFC production, and IM response of variously transduced cells were measured (n = 4). K, L WNT3 and the empty control (Ctrl) were delivered into the control (scramble) and ZFX-silenced CML CD34⁺ cells, CFC production and IM response of variously transduced cells were measured (n = 4). Data are presented as the mean ± SEM, and Student’s t-test was used to estimate the P-values (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001)