Fig. 7

ZFX silencing attenuates β-catenin signaling in BCR/ABL⁺ cells. A In K562 cells, the effects of ZFX silencing on several molecules in the Wnt signaling pathway were analyzed by western blotting, and representative graphs are shown (left). Cumulative data from the blots were analyzed statistically (right, n ≥ 3). B In BaF3–BCR/ABL cells, the expression of several molecules in the Wnt signaling pathway upon Zfx silencing was analyzed by western blotting (left), and cumulative data from the blots were subjected to statistical analysis (right, n ≥ 3). Data are presented as the mean ± SEM, and Student’s t-test was used to estimate the P-values (*P < 0.05 and **P < 0.01). C The proposed model illustrates the importance of the ZFX/WNT3 axis in CML stem/progenitor cells. The elevated ZFX promotes the transcription of WNT3, which sustains β-catenin signaling in CML stem/progenitor cells and confers growth advantage and drug resistance to these cells. FZD, Frizzled receptors