Fig. 2
Bacterially expressed recombinant EFR3A binds to recombinant flotillin-2 in vitro, in the overlay assay. Recombinant EFR3A protein was purified on metal ion chelating resin (Ni2+) and subjected to SDS-PAGE followed by transfer onto nitrocellulose. Then, membrane strips containing bands corresponding to molecular weight larger than 70 kDa were incubated with increasing concentrations of recombinant flotillin-2, followed by incubation with goat anti-flotillin-2 antibodies (Abcam) and secondary donkey anti-goat antibodies (Santa Cruz)