Fig. 6
Mobility of the raft probe in EFR3A KnD partially returns towards normal in “rescue”-transfected EFR3A KnD HeLa cells svFCS measurements. A Diffusion time as a function of waist surface area. Black line: control HeLa “scrambled”, red line: EFR3A KnD, and green line: “rescue”-transfected EFR3A KnD HeLa cells. Cells were grown in LabTek chambers in DMEM medium with 10% FBS. After 24 h cells were washed three times and stained with 0.075 μM BODIPY-SM in HBSS with 10 mM HEPES and 0.075 μM BSA medium for 10 min. Cells were washed three times and measurements were performed in HBSS buffer with 10 mM HEPES, pH 7.4. For comparison, wild-type cells were treated with 8 mM β-methyl cyclodextrin for 1 h at 37 °C B. T0 and apparent diffusion coefficient. SEM, standard error of the mean