Fig. 7

Silencing of EFR3A gene expression affects G1 and G2/M length and proliferation but not cell mobility. A Cell cycle phase distribution in HeLa cells was evaluated via PI staining followed by flow cytometry presented as histograms. B Quantitation of flow cytometry analysis. Error bars represent the SEM of three independent experiments. *p < 0.05. The percentage of G1 phase cells increased within 48 h to 56%. C Cell proliferation was evaluated by WST-1 staining according to the Methods section. **Indicates a p ≤ 0.01 compared with control cells. D Representative images from the wound healing assay showing changes in HeLa cells with decreased EFR3A gene expression in comparison with control “scrambled” cells under stimulation with EGFR (50 ng/ml) for 48 h. E Quantitative analysis from the wound healing assay in the form of a bar graph showing the ratio of wound closure in EFR3A KnD versus “scrambled” cells. The observed reduced motility in cells with decreased expression of EFR3A in comparison with “scrambled” cells was found to be statistically insignificant (p-value = 0.158)