Fig. 8

Silencing of EFR3 gene expression affects PLCγ1 and EGFR phosphorylation. A Effect of EFR3A gene silencing on activation of EGF-induced EGF receptor and phospholipase PLCγ1 in HeLa cells via western blotting analysis. B Effect of MβCD on EGFR and PLCγ1 phosphorylation upon EGF treatment of WT HeLa cells. Fifteen micrograms of cell lysates was loaded on the 10% SDS-PAGE gel. C and D Quantitative analysis of data presented in A as bar graphs of fold change calculated as the ratio of relative levels of phospho-PLCγ and pEGFR normalized to the corresponding PLCγ1 and EGFR signal respectively. Significance values were calculated by paired Student’s t-test for **p < 0.01, ***p < 0.001 and ****p < 0.0001