Table 1 Antibodies used in this project
Antibodies | Experiment |
|---|---|
Rabbit anti-EFR3A (Abnova GmbH, Taipei City, Taiwan) | WB 1:1000 |
Mouse anti-flotillin-2 (Santa Cruz Biotechnology, Dallas, TX, USA) | WB 1:1000; IP: 5 μg |
Mouse anti-FLAG (Merck KGaA, Darmstadt, Germany) | WB 1:1000 |
Rabbit anti-EGFR (Cell Signaling, Danvers, MA, USA) | WB 1:1000 |
Mouse anti-flotillin-1 (Santa Cruz Biotechnology, Dallas, TX, USA) | WB 1:1000 |
Goat anti-flotillin-2 (Abnova GmbH, Taipei City, Taiwan) | WB 1:1000; IP: 5 μg |
Mouse anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA) | WB 1:1000 |
Goat IgG (Santa Cruz Biotechnology, Dallas, TX, USA) | IP: 5 μg |
Mouse IgG (Santa Cruz Biotechnology, Dallas, TX, USA) | IP: 5 μg |
Anti-rabbit IgG (HRP) (Jackson ImmunoResearch, Ely, Cambridgeshire, United Kingdom) | WB 1:10,000 |
Anti-mouse IgG (HRP) (Jackson ImmunoResearch, Ely, Cambridgeshire, United Kingdom) | WB 1:10,000 |
Anti-goat IgG (HRP) (Santa Cruz Biotechnology, Dallas, TX, USA) | WB 1:10,000 |
Rabbit polyclonal anti-phospho-PLCγ1 Y783 (Cell Signaling, Danvers, MA, USA) | WB 1:1000 |
Rabbit polyclonal anti-PLCγ1 (Cell Signaling, Danvers, MA, USA) | WB 1:1000 |
Rabbit monoclonal phospho-EGF Receptor (Tyr1068) (D7A5) (Cell Signaling, Danvers, MA, USA) | WB 1:1000 |
Rabbit monoclonal EGF Receptor (D38B1) (Cell Signaling, Danvers, MA, USA) | WB 1:1000 |