Fig. 1

Cadmium inhibits mitochondrial transfer between AML12 cells. AML12 cells were treated with 5 μM Cd for 6 h. Live cell imaging of mitochondria (A), one group of CFDA-SE prestained cells was co-cultured with another population of Mito-tracker-Red prestained cells for confocal microscopy (B) Scale bar = 25 μm. Mito-tracker-Red with cell brightfield dual channel observation of mitochondrial transfer (C). Scale bar = 50 μm. AML12 cells were treated with 2.5, 5, and 10 μM Cd for 6 h, Scanning electron microscopy was used to observe intercellular tunneling nanotube structures (D). Scale bar = 50 μm. The Transwell co-culture system was used to detect the method of mitochondrial transfer (E, F). Scale bar = 50 μm. Confocal 3D imaging to detect intercellular mitochondrial transfer (G) Scale bar = 20 μm