Fig. 2
From: Cadmium promotes nonalcoholic fatty liver disease by inhibiting intercellular mitochondrial transfer
Mitochondrial transfer is bidirectional and associated with microtubules. AML12 cells were treated with 5 μM Cd for 12 h. One group of CFDA-SE prestained cells was co-cultured with another population of Mito-tracker-Red prestained cells for Flow cytometry to detect intercellular mitochondrial transfer efficiency (A–C). AML12 cells were treated with 2.5, 5, and 10 μM Cd for 6 h. Confocal microscopy was used to observe colocalization of phalloidin and Mito-tracker-Red (D). Scale bar = 25 μm. Tubulin-tracker and phalloidin co-staining to observe the effect of Cd on the cytoskeleton of AML12 cells (E). Scale bar = 25 μm. Confocal microscopy was used to observe colocalization of Tubulin-tracker-Red and Tomm20 (F). Scale bar = 25 μm