Fig. 4

Disruption of microtubules inhibits mitochondrial transfer and promotes lipid accumulation. AML12 cells were exposed to 5 μM Cd and/or 2 μM Noc for 6 h. Brightfield (BF) microscopy was used to detect the effect of Cd on cell morphology (A). Scale bar = 100 μm. Tubulin-Tracker-Red and phalloidin co-staining were used to observe the cytoskeleton (B). Scale bar = 25 μm. Immunofluorescence was used to observe mitochondrial morphology (C). Scale bar = 25 μm. Oil red O (D) and BODIPY (E) were used to observe intracellular lipid droplets. TG and TC contents were detected (F, G). Levels of CPT1and ACSL1 were determined using western blotting (H). Results are shown as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01. Compared with the Cd group, ^#P < 0.05, ^##P < 0.01