Fig. 7
From: Cadmium promotes nonalcoholic fatty liver disease by inhibiting intercellular mitochondrial transfer
P62-Dynaction1-driven negative mitochondrial transport mediates cadmium-induced inhibition of mitochondrial transfer. AML12 cells were treated with 2.5, 5, and 10 μM Cd for 6 h. Levels of Dynactin 1, P62, and DYNLL1 were determined using western blotting (A). Results are shown as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01. P62, Tubulin-Tracker-Red, and phalloidin co-staining (B). Scale bar = 25 μm. Co-staining of P62 and Dynactin1 was observed (C). Scale bar = 25 μm. AML12 cells were treated with 5 μM Cd for 6 h. Co-immunoprecipitation detection of Dynactin1 and DYNLL1 interactions (D). Co-immunoprecipitation detection of Dynactin1 and P62 interactions (E). Based on treatment with Dynactin1 siRNA and P62 siRNA for 24 h, AML12 cells were treated with or without Cd for 6 h. TOMM20 and Tubulin-Tracker-Red co-staining to detect mitochondrial transfer (F, G). Scale bar = 25 μm