Fig. 8
From: Cadmium promotes nonalcoholic fatty liver disease by inhibiting intercellular mitochondrial transfer
Inhibition of P62 alleviates cadmium-induced lipid accumulation. Based on treatment with SQSTM1 (P62) siRNA or NC siRNA for 24 h, AML12 cells were treated with or without Cd for 6 h. Oil red O (A) and BODIPY (B) were used to observe intracellular lipid droplets. Scale bar = 200 μm. Scale bar = 25 μm. TG and TC contents were detected (C, D). Levels of CPT1and ACSL1 were determined using western blotting (E). Results are shown as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01. Compared with the Cd group, #P < 0.05, ##P < 0.01