Fig. 9

Exogenous mitochondria relieve cadmium-induced cell damage. AML12 cells were exposed to 5 μM Cd for 6 h. Subsequently, they were co-cultured with mitochondria for 12 h, and was used CCK8 to detect cell activity (A). After mitochondrial pretreatment for 6 h, AML12 cells were exposed to 5 μM Cd for 6 h. CCK8 detection of cell activity (B). Compared with the control group, *P < 0.05, **P < 0.01. Compared with the Cd group, ^#P < 0.05, ^##P < 0.01. After mitochondrial pretreatment for 6 h, AML12 cells were exposed to 5 μM Cd for 6 h. Oil red O (C) and BODIPY (D) were used to observe intracellular lipid droplets. Scale bar = 200 μm. Scale bar = 25 μm. Levels of CPT1and ACSL1 were determined using western blotting (E). TG and TC contents were detected (F, G). Results are shown as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01. Compared with the Cd group, ^#P < 0.05, ^##P < 0.01. AML12 cells were treated with 2, 4, and 8 mM ATP for 6 h. CCK8 was used to detect cell activity (H). ATP pretreatment 6 h, AML12 cells were exposed to 5 and 10 μm Cd for 6 h, and CCK8 detected cell activity (I, J). Compared with the control group, *P < 0.05, **P < 0.01. Compared with the Cd group, ^#P < 0.05, ^##P < 0.01. After ATP pretreatment for 6 h, AML12 cells were exposed to 5 μM Cd for 6 h. Oil red O (K) and BODIPY (L) were used to observe intracellular lipid droplets. Scale bar = 200 μm. Scale bar = 25 μm. Levels of CPT1and ACSL1 were determined using western blotting (M). TG and TC contents were detected (N, O). Results are shown as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01. Compared with the Cd group, ^#P < 0.05, ^##P < 0.01