Fig. 6

Suppressing S403 phosphorylation protects cells from proteasome inhibition-induced cell death. A SQSTM1 knockout AD293 cells stably re-expressing FLAG-SQSTM1(WT or mutants), were treated with 2 μM MG132 for 36, and then examined the cell viability with CCK-8 assay. B AD293 cells were treated with MG132 (1 μM), Doramapimod (25 μM), and CX-4945 (1 μM), alone or in combination for 24 h, the cell viability was examined with CCK-8 assay. C, D AD293 cells were treated with Bortezomib (20 nM), Doramapimod (25 μM), and CX-4945 (1 μM), alone or in combination for 24 h, and then the images were acquired by brightfield microscopy (C), the cell viability analyzed with CCK-8 assay (D). Scale bar: 100 μm. For A, B and D, data are mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01