Fig. 1

Expression of miR-155-5p increases in iHvKp-Induced mouse model of AL. A Lung tissue identification. The inflammatory response in lung tissues was determined by HE staining after iHvKp injection 12 h via the tail vein. B Recruitment of inflammatory cells assessed by MPO activity assay. C Lung permeability was assessed by measuring BALF total protein concentration using a BCA assay. D Levels of pro-inflammatory cytokines TNF-α and IL-6 measured by RT-PCR. E Immunofluorescence intensity of macrophage polarization cell markers in lung tissues, iNOS (green, M1) and Arg1 (red, M2). F Expression of miR-155-5p in lung tissues measured by RT-PCR. G Levels of pro-inflammatory cytokines TNF-α and IL-6 in iHvKp-stimulated RAW264.7 macrophages measured by RT-PCR. H Level of M1 polarization determined by flow cytometry. The data are presented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, n = 3