Fig. 1

GCN5L1 gene KO impaired surfactant production in MLE-12 cells. A Phospholipid secretion in WT and GCN5L1-KO MLE-12 cells. B ELISAs of secreted SPs in the culture supernatant of WT and GCN5L1-KO MLE-12 cells. C Reconstruction of GCN5L1 expression repaired phospholipid secretion in GCN5L1 mutant cells. D Reconstruction of GCN5L1 expression repaired SP-B and SP-C secretion in GCN5L1 mutant cells. The results are expressed as the mean ± SD of three independent experiments; **P < 0.01; t-test