Fig. 2

Disruption of GCN5L1 altered the expression of Cebpα and surfactant-related genes. A Q-PCR verification of the surfactant-related DEGs in GCN5L1 mutant clones. B Q-PCR detection of Cebpα mRNA expression in WT and GCN5L1-KO MLE-12 cells. C Representative immunoblot image of Cebpα protein expression levels in WT and GCN5L1-KO MLE-12 cells. D Representative immunoblot images of Cebpα protein levels in WT cells, M2 cells, M2 cells infected with lentivirus expressing exogenous Cebpα (M2 + Cebpα), and M2 cells infected with lentivirus expressing exogenous GCN5L1 (M2 + GCN5L1). E Q-PCR assays of the RNA levels of surfactant-related genes in WT cells, M2 cells, and M2 cells infected with lentivirus expressing exogenous Cebpα (M2 + Cebpα), and M2 cells infected with lentivirus expressing exogenous GCN5L1 (M2 + GCN5L1). The results are expressed as the mean ± SD of three independent experiments; ns, not significant;*P < 0.05, **P < 0.01; t-test