Fig. 4

Mitochondria-localized GCN5L1 is responsible for the regulation of surfactant-related genes. A Colocalization of GCN5L1–FLAG with TOM20 in MLE-12 cells. Cells were cotransfected with TOM20- and GCN5L1–FLAG-expressing plasmids before staining with anti-TOM20 and anti-FLAG antibodies. B The colocalization scatter plot of anti-TOM20 and anti-GCN5L1–FLAG signals. Pearson correlation coefficient is 0.78, Manders’ overlap coefficient is 0.82. C Q-PCR assays of the RNA levels of surfactant related genes in WT cells, M2 cells, and M2 cells infected with lentivirus expressing mito-GCN5L1 (M2 + mito-GCN5L1).The results are expressed as the mean ± SD of three independent experiments; *P < 0.05, **P < 0.01; t-test