Fig. 5

Impairment of LB positioning/trafficking in GCN5L1-KO cells. A Lysotracker Red staining of WT, GCN5L1-KO clones (M2 and M29), and GCN5L1 reconstructed mutant cells (M2 + GCN5L1). B WT and M2 cells were metabolically labeled with BODIPY phosphatidylcholine. C WT and M2 cells were immunostained with Lamp1 antibody. D–F WT and M2 cells were transfected with h-ABCA3–EGFP or ABCA3–mCherry before staining with Lysotracker Red (D), BODIPY phosphatidylcholine (E), and Lamp1 antibody (F)