Fig. 2

dCas9-KRAB gene expression in prostate cancer cells. A RNA expression levels of dCas9-KRAB in four prostate cancer cell lines and one prostate cell line. B RNA expression levels of PSA mRNA in four prostate cancer cell lines and one prostate cell line. C dCas9-KRAB/sgRNA targeting the PSA ORF region locally forms heterochromatin and blocks the sliding of RNA polymerase on the DNA strand. **P < 0.01 (Student’s t-test, n = 3 replicates)