Fig. 3

Periostin overexpression worsened cardiac injury and dysfunction in diabetic mice. A Representative blots and quantitation of periostin protein. B, C Left ventricle EF and FS were quantified. D Representative echocardiographic images showing the effects of perisotin knockdown on cardiac function in control and diabetic mice. E Serum LDH levels in mice. F Serum CK-MB levels in mice. G Representative photographs of the myocardium with H&E staining (Scale bar = 100 μm). H Representative photographs of the myocardium with Sirus red staining (Scale bar = 100 μm). I Representative images of the myocardium with DHE staining (Scale bar = 200 μm). J Heatmap showing the mRNA levels of pro-hypertrophic genes (ANP, BNP, β-MHC) and pro-fibrogenic genes (Col I, Col III, α-SMA). K Heatmap showing the mRNA levels of pro-inflammtory genes (IL-1β, IL-6, TNF-α) and pro-oxidative genes (NOX2, NOX4, 12-LOX). n = 4–6. *P < 0.05 versus Control (Con), †P < 0.05 versus Diabetes. Differences between groups were assessed with ANOVA followed by Bonferroni post-hoc test