Fig. 4

Periostin knockdown attenuated, while periostin overexpression intensified HG-induced CF activation. A, B Representative photographs and quantitative analysis of EdU-positive cells. C Cell viability. D MDA contents. E, F Representative photographs and quantitative analysis of DHE staining. G Representative blots and quantitation of α-SMA and collagen I. H Representative mRNA levels of TNF-α and IL-1β. I, J Representative photographs and quantitative analysis of EdU-positive cells. K Cell viability. L MDA contents. M, N Representative photographs and quantitative analysis of DHE staining. O Representative blots and quantitation of α-SMA and collagen I. P Representative mRNA levels of TNF-α and IL-1β. n = 4–6. *P < 0.05 versus Control (Con), †P < 0.05 versus HG. Differences between groups were assessed with ANOVA followed by Bonferroni post-hoc test