Fig. 5

RNA sequencing revealed that perisotin was a diver of NAP1L2 in CF. A Heatmap showing the differential gene cluster analysis between control heart and periostin OE hearts. B Volcano map showing the overall distribution of differentially expressed genes between control heart and periostin OE hearts. C The differential gene expression level radar graph showed the 30 up/down genes with the lowest Q or P values. D The mRNA levels of top 10 upregulated genes in mouse hearts with periostin OE. E The protein expression of in CF exposed to NG or HG using western blot. F Effects of periostin siRNA on the protein expression of NAP1L2. G Effects of periostin OE on the protein expression of NAP1L2. H–J Representative blots and quantitation of α-SMA and collagen I. K Cell viability. L MDA contents. M Representative mRNA levels of TNF-α and IL-1β. n = 4–6. *P < 0.05 versus Vehicle (Veh), Con or NG, †P < 0.05 versus HG. The P-value was calculated by unpaired two-tailed Student’s t-test (D, E). Differences between groups were assessed with ANOVA followed by Bonferroni post-hoc test (F–M)