Fig. 1

Reaction specificity of wildtype and mutant recombinant mouse Alox15 with different polyenoic fatty acids. Wildtype and mutant (Leu353Phe) mouse Alox15 were expressed as N-terminal his-tag fusion proteins as described in the Additional file 1 and aliquots of the bacterial lysis supernatants were used as enzyme source. After a 5 min incubation period with different polyenoic fatty acids (100 µM) the reaction products were analyzed by RP-HPLC. A AA oxygenation products. B Triad Concept cartoon explaining AA 12-lipoxygenation by wildtype mouse Alox15 (see text for detailed explanation). C Triad Concept cartoon explaining AA 15-lipoxygenation by the Leu353Phe mutant of mouse Alox15 (see text for detailed explanation). D EPA oxygenation products, E dihomo-gamma-linoleic acid oxygenation products, F DHA oxygenation products, G alpha-linolenic acid oxygenation products, H linoleic acid oxygenation products. Insets: UV-spectra of the major conjugated dienes peak labeled by small letters. Representative chromatograms (n ≥ 4) are shown