Fig. 2

Expression of Alox15 mRNA in different mouse cells and knock-in strategy for generation of humanized Alox15 knock-in mice. A Total RNA was extracted from different cells and tissues of wildtype mice, the RNA was reversely transcribed and the Alox15 mRNA steady state concentrations were quantified by qRT-PCR (see Additional file 1: Methodological supplement). Two independent measurements (n = 2) were carried out for each cDNA sample. B Crisp/Cas9 strategy for generation Alox15-KI mice. C Quantitative RT-PCR of Alox-isoforms in peritoneal lavage cells prepared from Alox15-KI mice and outbred wildtype control animals. Expression levels of Alox15 and Alox5 mRNA in wildtype cells were separately set 100%. Experimental details are given in the Additional file 1: Methodological supplement