Fig. 3

Genomic sequencing and ex vivo Alox activity assays. A–C The mutated region of the Alox15 gene was amplified by PCR and the amplification products were sequenced (for methodological details see Additional file 1). D–F Ex vivo Alox15 activity assays (see Additional file 1) using peritoneal lavage cells as enzyme source. Representative partial RP-HPLC chromatograms are shown. G, I Ex vivo Alox15 activity assays (see Additional file 1) using bone marrow cells as enzyme source. H Summary and statistical evaluation (means ± SD, n = 4) of the Alox15 activity assays. J Ex vivo Alox5 activity assays (n = 4) using whole blood as enzyme source. Leukotriene B4 formation was quantified as readout parameter