Fig. 3

CircPan3 interacts with miR-320-3p. A A conserved miR-320-3p binding site exists in the circPan3 sequence. Minimum free energy (mfe) was calculated by RNAhybrid. B MiR-320-3p is captured by circPan3. The levels of captured miR-320-3p and U6 by the biotin-labelled circPan3 probe or scrambled control probe (Random) were analyzed by qPCR. The relative pellet/input ratios were calculated. **P < 0.01. n = 3. C CircPan3 is captured by miR-320-3p. The levels of captured circPan3 and GAPDH by biotin-labelled miR-320-3p mimics or negative control mimics (Mimic-NC) were analyzed by qPCR. The relative pellet/input ratios were calculated. **P < 0.01. n = 3. D Overexpression of circPan3 does not affect miR-320-3p levels in cardiomyocytes. Cardiomyocytes were transfected with the circPan3 expression vector or empty vector. The expression level of miR-320-3p was analyzed by qPCR. n = 3. E Knockdown of circPan3 does not affect miR-320-3p levels in cardiomyocytes. Cardiomyocytes were transfected with siRNAs. The expression level of miR-320-3p was analyzed by qPCR. n = 3