Fig. 4

Bioinformatic analysis of differentially expressed tsRNAs (DE-tsRNAs) and target gene screening. A Potential target genes of DE-tsRNAs were predicted by miRanda and TargetScan. The clustering map of DE-tsRNAs and target genes was generated by Cytoscape. B The process of screening for target genes that may be involved in intimal hyperplasia. First, the GeneCard database was used to select genes associated with intimal hyperplasia from the predicted list of potential target genes. Then, a GEO dataset GSE164050 (n = 4) was used to screen the genes downregulated in the balloon injury rat model, and 4 genes GLP1R, FMOD, HSPB6 (HSP20), and XBP1 were focused. C According to GSE164050, the mRNA expression of FMOD and HSP20 was decreased in the balloon injury, the expression of XBP1 was undetectable, and the expression change of GLP1R was not statistically significant. D Carotid proteomic detection confirmed that the protein levels of FMOD and HSP20 were decreased in the balloon injury group. n = 3, data are expressed as mean ± SD. *P < 0.05, **P < 0.01; ns indicates not statistically significant