Fig. 6

tRF-Glu-CTC inhibited fibromodulin (FMOD) levels in vascular smooth muscle cells (VSMCs) and upregulated VSMC proliferation and migration. A According to bioinformatics analysis, tRF-Glu-CTC may be a negative regulator of fibromodulin (FMOD) in VSMCs. Therefore, polynucleotide analogs of tRF-Glu-CTC were synthesized and transfected into rat thoracic aortic VSMCs by liposomes. B Compared with the transfection of scrambled RNA sequences, the transfection of tRF-Glu-CTC analog inhibited the level of FMOD in VSMCs. C Transfection of tRF-Glu-CTC increased the proliferation of VSMCs in the EdU incorporation assay. D Transfection of tRF-Glu-CTC increased the migration of VSMCs in the wound healing assay. E Overexpression of tRF-Glu-CTC also increased the migration of VSMCs in the wound healing assay. n = 5, data are expressed as mean ± standard error, *P < 0.05, **P < 0.01