Fig. 8

Transfection of tRF-Glu-CTC antisense attenuated neointimal formation after carotid artery balloon injury in rats. A Multipoint injection of chemically modified tRF-Glu-CTC antisense around the arteries on the side of surgery and repeated every 3 days until postoperative day 14 after carotid balloon injury in rats. B Detection of tRF-Glu-CTC expression in rat carotid arteries at 14 days. Compared with the control group injected with scrambled sequence, the expression of tRF-Glu-CTC in the carotid artery of the tRF-Glu-CTC antisense injection group decreased. C The cross section of the rat carotid artery showed that the area of carotid intimal hyperplasia was less in the tRF-Glu-CTC antisense treatment group than in the control group. The intima/media ratio was decreased in the tRF-Glu-CTC antisense treatment group. D Immunohistochemical staining for FMOD on rat carotid arteries showed that rats transfected with tRF-Glu-CTC antisense increased the positive staining of FMOD in the vessel wall after carotid artery intima injury compared with the control group transfected with scrambled RNA sequence. E Western blotting showed the increased level of FMOD in the rat carotid artery transfected with tRF-Glu-CTC antisense after balloon injury. F Transfection of tRF-Glu-CTC antisense decreased the level of Smad3, the major effector of TGF-β1. n = 5, data are expressed as mean ± standard error, *P < 0.05, **P < 0.01