Fig. 1

Evolutionary analysis of R-RAS subfamily proteins. A Phylogenetic relationships of R-RAS subfamily proteins in Metazoa and Protista. The representatives of R-RAS2-like and R-RAS2 proteins are highlighted in purple, with corresponding taxonomic groups indicated. The R-RAS and M-RAS proteins are shaded in grey. ML bootstrap values, based on 1000 bootstrapping replications, are represented as numbers associated with the branches (bootstrap values higher than 50% are displayed at the branching points). The scale bar denotes the number of substitutions per site. Additional file 2: Table S1 provides the accession numbers of the amino acid sequences used in our study. B Multiple sequence alignment of R-RAS2-like proteins from sponges and human R-RAS subfamily members. Conserved domains are indicated as follows: G motifs are shown in purple above the alignment, Switch regions in blue below the alignment, hypervariable region (HVR) in red below the alignment, and CaaX box in green above the alignment. C A schematic representation of human R-RAS2 protein with indicated conserved domains (above) and D the structure of r-ras2 and r-ras2-like genes from selected metazoans (below). Triangles mark the positions of introns, and the number within each triangle represents the intron phase. Black dashed lines connect introns that share the same positions and phases based on the alignment of amino acid sequences. The sequences of genes with corresponding intron positions were obtained from the NCBI's genomic database. E A table showing the number of R-RAS subfamily members among different species