Fig. 5
From: CRISPR–Cas9 applications in T cells and adoptive T cell therapies
Applications of CRISPR–Cas9 screening system. A CRISPR screens can be applied to various contexts, including primary T cells, engineered T cells, and cell lines, as well as various diseases. These applications are not limited to hematologic and solid cancers but also extend to infectious and inflammatory diseases. B At the cellular level, CRISPR screens can elucidate the roles of transcription factors in determining cell fates and differentiation, and they can identify regulators of cell migration, metabolism, and cell exhaustion/activation. In the context of hematologic and solid cancers, CRISPR screens can identify cancer-associated antigens and factors influencing immunotherapy resistance. For nontumor diseases such as COVID-19 and coronary artery disease (CAD), CRISPR screens can help understand coronaviruses’ pathogenic mechanisms and identify disease risk factors. At the organoid level, CRISPR screens can be employed to identify susceptibility genes and serve as a tool for drug screening and mechanism exploration