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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Sialylation on vesicular integrin β1 determined endocytic entry of small extracellular vesicles into recipient cells

Fig. 2

Effects of sialylation on sEV entry into recipient cells. A Sialic acid levels on sialidase-treated sEV. CBB: Coomassie Brilliant Blue staining. B The particle size and particle count of YTS-1 sEV and desialylated sEV determined by nanoparticle tracking analysis (NTA). C The fluorescence signal of sialylated and desialylated sEV labeled by ExoTracker determined by flow cytometry. D Effect of sialylation on sEV uptake, analyzed by flow cytometry. E Levels of biotin-labeled vesicular proteins in recipient cells. F Y/GPI-NEU1 cell construct (schematic). G The expression of GPI anchored NEU1 in Y/GPI-NEU1 cells, analyzed by western blotting. Tubulin was used as loading control. H The expression of GPI anchored NEU1 in Y/GPI-NEU1 sEV. TSG101 was used as loading control. I Sialic acid levels in Y/GPI-NEU1 sEV. TSG101 was used as loading control. J Uptake of Y/GPI-NEU1 sEV and Y-vec sEV by HCV29. K Uptake of Y/GPI-NEU1 sEV and Y-vec sEV by HCV29 and sialidase pre-treated HCV29

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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