Fig. 3

Western blot and relative densitometries of FASN (a, b), ACSS1 (a, c), and precursor and cleaved SREBP1 (a, d, e) expression in Oli-Neu cells; GAPDH was used for endogenous normalization. Confocal microscopy images (f) in Oli-Neu cells; nuclei were labelled with DAPI. Scale bar, 20 µm; 100× objective. Reverse transcription real-time PCR analysis (g). GAPDH were used as endogenous controls. Values are mean ± standard deviation (SD) of at least three independent experiments; ***p < 0.001, **p < 0.01, *P < 0.05, compared with control Oli-Neu; Student’s t-test