Fig. 5

Master regulator analysis of PROX1 and SMARCC2 in two different networks (frontal cortex and hippocampus) showing the most significant transcription factors with differentially activated networks in kdAGC1 versus control (a). The upper bar shows the symbol of the tested master regulator, its normalized enrichment score (NES), with its cell colored in red for activated regulons and blue for downregulated regulons, and the associated adjusted p value. The barcode graph indicates the distribution of activated (red bars) and repressed (blue bars) targets of a master regulator. Target genes are ranked from left to right from most downregulated to most upregulated according to kdAGC1 versus control signature. The plot on the right shows the most significantly altered targets of a master regulator’s regulon, with a line indicating the master regulator as an activator (arrowhead) or a repressor (blunted end) and the color on the target showing whether it is upregulated (red) or downregulated (blue) in the signature. Local splicing variations (LSVs) graph of PMP22 and RARG showing on the left the splicing events relative to a specific exon and on the right the values of the most relevant events’ ΔPSI values (b). Violin plots express the expected dPSI values, with values above 0 representing a prevalence of the splicing event in kdAGC1 samples, while values below 0 represent a prevalence in control samples. Bar graph showing concentrations (ng/mL) and relative error bars in kdAGC1 and control samples. Metabolites are sorted left to right according to increasing p values (*p < 0.05; c). Significance levels are reported with asterisks. Reported p values were corrected using the Benjamini–Hochberg method. Separated analysis and graphs of metabolites quantification for cell pellets and media are in Additional file 5: Figs. S5 and S6