Table 1 Target genes and primers for real time PCR analysis
Gene | Primers (5′–3′) |
|---|---|
ACSS1 | F: AAGATTTCTGTGATGACGCTGG R: TCTGGGAAAGTGATGAGGAGAC |
ATPAF2 | F: TATCCTGCTGAGAGTCCCATTC R: GGCTTTGAGATAAACCTGGACC |
CHODL | F: TTCCGAAACTGGTACACTGATG R: GGGATGGAAATGGTCACCTTAC |
FASN | F: CCAGAGGGTGGTTGTTAGAAAG R: TCAACTCACTGGCAGAAGAGAA |
GAPDH | F: AGGGTGGTGAAGCAGGCATC R: CGAACGTGGAAGAGTGGGAG |
JMJD4 | F: ATGGTAACCTGCCCTATGATGT R: GGGTAACTTCAGTATGTCCTCG |
ME2 | F: TCTGAGGAGGTGTCAGTGAAGA R: AGAGAGAGTGCATAGACCGGAA |
NAT8L | F: TGCCATGCTGCACAACTACT R: AGATACTCAGTGACCCGAAGTC |
RAI1 | F: CCAGAATCTTCACGCTTACCAG R: TTTGTGAGGTGATGGTCTTGGA |
SLC25A13 | F: AGCTAGGTCGATTCCTGCATAG R: GAAGTGCAAGATTCTAGGCGAA |
SNAP47 | F: GAAGACCACATTTCGACTAGGC R: AGCGTGGCTTCTCATTTCTCTCT |
SREBP1 | F: TTGTTTGCGATGTCTCCAGAAG R: TGGCCAATGGACTACTAGTGTT |
SREBP2 | F: GGACAGTGATGTGGACTTGAAA R: GGGATAAGGTAACTGAGACTCG |
TRIM11 | F: GCCATCTCTCATTCTACAGTGC R: GGATACTGATAGACGTCCGACT |