Fig. 2

Chromatin accessibility analysis of the RM, PC, and CA tissue layers in antler growth center. A Genome-wide distribution of ATAC signals in the three layers. B Complex heatmap of ATAC signal matrix. All ATAC signals in the three layers were allocated into three clusters. C Genome browser view showing ATAC-seq signal around the pri-miR-140 loci. RM, PC, and CA represent three tissue layers, respectively. The yellow shadow denotes the ATAC signal that was correlated with miR-140 expression. The black arrow represents the direction of transcription. D Expression trend analysis of transcription factors in the upstream of miR-140-3p in the three layers. The red line represents the genes that were consistent with the expression trend of miR-140-3p. The blue line represents the genes that were opposite with the expression trend of miR-140-3p, and the line that represents Prrx1 is marked with the red arrow. E Expression heatmap of genes opposite with miR-140-3p; Prrx1 is marked with the red arrow. F Genome browser view showing CUT&Tag-seq signal around the pri-miR-140 loci. rep1, rep2, and rep3 represent three experimental repeats, respectively. RMCs represents the RM cells. The yellow shadow denotes the CUT&Tag signal that was correlated with miR-140-3p expression. The black arrow represents the direction of transcription