Fig. 2
From: miR-29b-3p regulates cardiomyocytes pyroptosis in CVB3-induced myocarditis through targeting DNMT3A
Pyroptosis increased in both in vivo and in vitro VMC models induced by CVB3. A The expressions of pyroptosis related proteins in heart tissue. B Primary cardiomyocytes isolated from SD rats were identified by immunofluorescence staining. The primary antibody of anti-cTnT was applied. The scale bar was 100 μm. C The total RNA was extracted from the primary cardiomyocytes exposed to varying concentrations of CVB3 and the expressions of inflammatory cytokines IL-1β and IL-18 were evaluated by qRT–PCR. D The cell viability was detected by CCK8. ####p < 0.0001 represents the comparison with control at 48 h; ****p < 0.0001 represents the comparison with control at 72 h. E, F The expressions of pyroptosis-related proteins in CVB3 treated primary cardiomyocytes and HL-1 cells were measured using western blotting. N = 3, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001