Fig. 5

Downregulation of miR-29b-3p-ameliorated, CVB3-induced VMC through decreasing pyroptosis in vivo. A A schematic diagram of miR-29b-3p antagomir treatment in VMC model induced by CVB3. The mice were inoculated with CVB3 on day 0 and then treated with indicated concentrations of miR-29b-3p antagomir (5, 10, 20 nM) on days 1, 3, and 5, respectively. B The body weight change of VMC mice after miR-29b-3p antagomir treatment. C M-mode images of heart and D–G the parameters of cardiac function were evaluated using echocardiography. H Heart appearance of VMC mice treated with miR-29b-3p antagomir on day 7. I H&E staining of the heart tissue sections; the magnifications were 1.25 × , 10 × , and 40 × , respectively, and the scale bar was 100 μm. J The inflammatory lesions were quantified using Image-Pro Plus 6.0 software. K Total RNA was extracted from heart tissue and the expression of miR-29b-3p was evaluated by qRT–PCR. L Total proteins were extracted from heart tissue and the expressions of pyroptosis proteins were analyzed by western blotting. N = 3, #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001; ns significance is annotated as not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001