Fig. 7

miR-29b-3p regulated cardiomyocyte pyroptosis via DNMT3A. The primary cardiomyocytes were exposed to CVB3 for 6 h, then co-transfected with phage-DNMT3A or phage-vector and miR-29b-3p inhibitor for another 48 h. A The expression levels of proteins related to pyroptosis. B, C Primary cardiomyocytes were transfected with 20 μM of si-DNMT3A or si n.c. for 48 h, then the expressions of DNMT3A mRNA and protein were measured. D The CVB3-infected cells were co-transfected with si-DNMT3A or si n.c. and miR-29b-3p inhibitor. A total of 48 h later, pyroptosis level of cardiomyocytes was evaluated by western blotting. N = 3, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001