Table 1 Primer sequences used in this study
From: miR-29b-3p regulates cardiomyocytes pyroptosis in CVB3-induced myocarditis through targeting DNMT3A
Name | Sequences |
|---|---|
miR-29b-3p-RT | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACACT-3′ |
miR-29b-3p-FP | 5′-CGCGTAGCACCATTTGAAATC-3′ |
miR-29b-3p-RP | 5′-AGTGCAGGGTCCGAGGTATT-3′ |
U6-RT | 5′-GTCGTATGCAGAGCAGGGTCCGAGGTATTCGCACTGCATACGACAAAATATGG-3′ |
U6-FP | 5′-AAGGATGACACGCAAATTC-3′ |
U6-RP | 5′-GAGCAGGGTCCGAGGT-3′ |
IL-6-FP | 5′-TAGTCCTTCCTACCCCAATTTCC-3′ |
IL-6-RP | 5′-TTGGTCCTTAGCCACTCCTTC-3′ |
IL-18-FP | 5′-GACTCTTGCGTCAACTTCAAGG-3′ |
IL-18-RP | 5′-CAGGCTGTCTTTTGTCAACGA-3′ |
DNMT3A-FP | 5′-GAGGGAACTGAGACCCCAC-3′ |
DNMT3A-RP | 5′-CTGGAAGGTGAGTCTTGGCA-3′ |
VP1-FP | 5′-TTGCATATGGCCCAGTGGAAG-3′ |
VP1-RP | 5′-TGTGGATCCTTATTGCCTAGTAGTGGTAACTC-3′ |
GAPDH-FP | 5′-AGGTCGGTGTGAACGGATTTG-3′ |
GAPDH-RP | 5′-TGTAGACCATGTAGTTGAGGTCA-3′ |
miR-29b-3p inhibitor | 5′-AACACUGAUUUCAAAUGGUGCUA-3′ |
miR-29b-3p mimic-sense | 5′-UAGCACCAUUUGAAAUCAGUGUU-3′ |
miR-29b-3p mimic-antisense | 5′-CACUGAUUUCAAAUGGUGCUAUU-3′ |
miR-29b-3p-antagomir | 5′- (mA)*(mA)*(mC)(mA)(mC)(mU)(mG)(mA)(mU) (mU)(mU) (mC)(mA) (mA)(mA) (mU)(mG) (mG)(mU) * (mG) * (mC) * (mU) * (mA)-3′ |
si-DNMT3A-sence | 5′-CCAGAACUGUAAGAACUGCUU-3′ |
si-DNMT3A-antisence | 5′-AAGCAGUUCUUACAGUUCUGG-3′ |