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The transcript expression profile of the leptin receptor-coding gene assayed with the oligonucleotide microarray technique — Could this be an Anorexia nervosa marker?

Abstract

Anorexia nervosa is a serious eating disorder with the highest mortality rate of any psychiatric disorder. The DSM-IV classification differentiates two AN types: the restricting type (AN-R) and the binge-eating/purging type (AN-BP). Leptin (LEP) levels can be thought of as a signal to the body of its energy reserves. The leptin receptor (including all its mRNA isoforms) is expressed in many tissues. Our aim was to discover the transcript expression profile of the LEP receptor-coding gene in the peripheral blood mononuclears in AN-R and AN-BP patients. Three young women suffering from Anorexia nervosa (one with AN-BP and two with AN-R) took part in the study, along with three non-anorexic subjects as our reference group. LEP receptor gene expression was examined using the oligonucleotide microarray method (HG-U133A, Affymetrix). The results were normalized using RMAExpress. Next, the accumulation analysis method was used (clustering). Hierarchical clustering resulted in three groups of separate clusters. The first group (cluster I) consisted of AN-R patients. The next group (cluster II) consisted of reference group patients suffering from different psychic disorders not related to eating disorders. Cluster III consisted of two patients — the first with AN-BP and the second with an adaptive disorder.

Abbreviations

DSM IV Classification:

Diagnostic and Statistical Manual of Mental Disorders

NCBI:

National Center for Biotechnology Information

AN:

Anorexia nervosa

AN-R:

restricting type of Anorexia nervosa

AN-BP:

binge-eating/purging type of Anorexia nervosa

LEP:

leptin

LEPR:

Homo sapiens leptin receptor

LEPROTL1:

Homo sapiens leptin receptor overlapping transcript-like 1

LEPROT:

leptin receptor overlapping transcript

OB-R:

Leptin receptor coding gene

OB-R:

leptin receptor

Ob-Ra:

leptin receptor splice variant

Ob-Rb:

leptin receptor splice variant

Ob-Rc:

leptin receptor splice variant

Ob-Rd:

leptin receptor splice variant

Ob-Re:

leptin receptor splice variant

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Correspondence to Małgorzata Janas-Kozik.

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Key words

  • Anorexia nervosa
  • Leptin receptor
  • Oligonucleotide microarray