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The identification and characterization of a testis-specific cDNA during spermatogenesis


Using bioinformatics and experimental validation, we obtained a cDNA (named srsf) which was exclusively expressed in the mouse testes. RT-PCR analysis showed that srsf mRNA was not expressed in the gonad during the sex determination period or during embryogenesis. In developing mouse tests, srsf expression was first detected on post-natal day 10, reached its highest level on day 23, and then reduced to and remained at a moderate level throughout adulthood. In situ hybridization analysis demonstrated that srsf mRNA was expressed in pachytene spermatocytes and round spermatids in the testes. The predicted protein contains one RNA-binding domain (RBD) and a serine-arginine rich domain (RS), which are characterized by some splicing factors of SR family members. These findings indicate that srsf may play a role during spermatogenesis.



type Aspermatogonia


intermediate spermatogonia


type B spermatogonia


preleptotene spermatocytes


leptotene spermatocytes


zygotene spermatocytes


early pachytene spermatocytes


late pachytene spermatocytes


diplotene spermatocytes


first meiosis


second meiosis


round spermatids


elongated spermatids


step 16 spermatids










round spermatid.


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Correspondence to Ping Song.

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Chen, Y., Hu, J., Song, P. et al. The identification and characterization of a testis-specific cDNA during spermatogenesis. Cell. Mol. Biol. Lett. 11, 80–89 (2006).

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Key Words

  • Expression pattern
  • Spermatogenesis
  • Splicing factor