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The assessment of DNA from marine organisms via a modified salting-out protocol

Cellular & Molecular Biology Letters volume 11, pages 155–160 (2006)Cite this article

Abstract

We developed a rapid, practical and non-toxic salting-out method for the extraction of DNA from marine organisms, and tested it on two representative species of Porifera and Cnidaria, both living in association with symbiotic zooxanthellae. We tested the efficiency of the protocol by comparing the output of the method for fresh tissue, frozen tissue and tissue stored in ethanol. It proved to be effective for extracting DNA in the case of the methods of preservation considered here, and for obtaining quantities of DNA comparable to those obtained via the traditional approach. The DNA from both species was of good quality. The DNA obtained was amplified by PCR using specific primers for the large ribosomal subunit, allowing the identification of the presence of both the host and symbiont genomes.

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Authors and Affiliations

  1. Dipartimento di Biologia, Università di Genova, Viale Benedetto XV, 16132, Genova, Italy

    Giovanni Battista Ferrara, Barbara Murgia, Anna Maria Parodi & Giulio Palmisano

  2. Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132, Genova, Italy

    Laura Valisano, Carlo Cerrano & Michele Sara

  3. Dipartimento di Scienze del Mare, Università Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy

    Giorgio Bavestrello

Authors
  1. Giovanni Battista Ferrara
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  2. Barbara Murgia
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  3. Anna Maria Parodi
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  4. Laura Valisano
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  5. Carlo Cerrano
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  6. Giulio Palmisano
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  7. Giorgio Bavestrello
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  8. Michele Sara
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Correspondence to Carlo Cerrano.

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Ferrara, G.B., Murgia, B., Parodi, A.M. et al. The assessment of DNA from marine organisms via a modified salting-out protocol. Cell. Mol. Biol. Lett. 11, 155–160 (2006). https://doi.org/10.2478/s11658-006-0013-7

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  • DOI: https://doi.org/10.2478/s11658-006-0013-7

Key words

  • Symbiodinium
  • Porifera
  • Cnidaria
  • DNA
  • PCR

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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