Open Access

The assessment of DNA from marine organisms via a modified salting-out protocol

  • Giovanni Battista Ferrara1,
  • Barbara Murgia1,
  • Anna Maria Parodi1,
  • Laura Valisano2,
  • Carlo Cerrano2Email author,
  • Giulio Palmisano1,
  • Giorgio Bavestrello3 and
  • Michele Sara2
Cellular & Molecular Biology LettersAn International Journal200611:13

https://doi.org/10.2478/s11658-006-0013-7

Received: 21 December 2005

Accepted: 21 February 2006

Published: 01 June 2006

Abstract

We developed a rapid, practical and non-toxic salting-out method for the extraction of DNA from marine organisms, and tested it on two representative species of Porifera and Cnidaria, both living in association with symbiotic zooxanthellae. We tested the efficiency of the protocol by comparing the output of the method for fresh tissue, frozen tissue and tissue stored in ethanol. It proved to be effective for extracting DNA in the case of the methods of preservation considered here, and for obtaining quantities of DNA comparable to those obtained via the traditional approach. The DNA from both species was of good quality. The DNA obtained was amplified by PCR using specific primers for the large ribosomal subunit, allowing the identification of the presence of both the host and symbiont genomes.

Key words

SymbiodiniumPoriferaCnidariaDNAPCR

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