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Brefeldin a decreases the activity of the general amino acid permease (GAP1) and the more specific systems for L-leucine uptake in Saccharomyces cerevisiae

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Abstract

Brefeldin A is a commonly used antifungal agent that reversibly blocks protein transport from the endoplasmic reticulum to the Golgi complex. In this study, we aimed to characterize L-leucine uptake in Saccharomyces cerevisiae in the presence of brefeldin A. For this purpose, we used a synthetic medium, containing L-proline and the detergent SDS, which allows the agent to permeate into the yeast cell. The results obtained with a wild type strain and a gap1 mutant indicate that BFA causes either direct or indirect modification of the transport and/or processing of L-leucine permeases. The presence of BFA affects the kinetic parameter values for L-leucine uptake and decreases not only the uptake mediated by the general system (GAP1), but also that through the specific BAP2 (S1) and/or S2 systems.

Abbreviations

AAP:

amino acid permease

BAP2:

branched-chain amino acid permease 2

BFA:

brefeldin A

ER:

endoplasmic reticulum

GAP1:

general amino acid permease

GC:

Golgi complex

S1 and S2:

specific L-leucine transport systems

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Correspondence to Carlos A. Stella.

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Key words

  • Brefeldin A
  • Leucine permeases
  • Saccharomyces cerevisiae