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Brefeldin a decreases the activity of the general amino acid permease (GAP1) and the more specific systems for L-leucine uptake in Saccharomyces cerevisiae

Cellular & Molecular Biology Letters volume 11, pages 256–263 (2006)Cite this article

Abstract

Brefeldin A is a commonly used antifungal agent that reversibly blocks protein transport from the endoplasmic reticulum to the Golgi complex. In this study, we aimed to characterize L-leucine uptake in Saccharomyces cerevisiae in the presence of brefeldin A. For this purpose, we used a synthetic medium, containing L-proline and the detergent SDS, which allows the agent to permeate into the yeast cell. The results obtained with a wild type strain and a gap1 mutant indicate that BFA causes either direct or indirect modification of the transport and/or processing of L-leucine permeases. The presence of BFA affects the kinetic parameter values for L-leucine uptake and decreases not only the uptake mediated by the general system (GAP1), but also that through the specific BAP2 (S1) and/or S2 systems.

Abbreviations

AAP:

amino acid permease

BAP2:

branched-chain amino acid permease 2

BFA:

brefeldin A

ER:

endoplasmic reticulum

GAP1:

general amino acid permease

GC:

Golgi complex

S1 and S2:

specific L-leucine transport systems

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, University of Buenos Aires, Paraguay, 2155, 5° Piso, Argentina

    Manuel Alonso, Hilda I. Burgos, Vanesa Pannunzio, Andrea Monti Hughes & Carlos A. Stella

  2. Biotechnology Center, University of Colorado, Colorado Springs, CO, USA

    James R. Mattoon

Authors
  1. Manuel Alonso
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  2. Hilda I. Burgos
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  3. Vanesa Pannunzio
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  4. Andrea Monti Hughes
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  5. James R. Mattoon
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  6. Carlos A. Stella
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Corresponding author

Correspondence to Carlos A. Stella.

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Alonso, M., Burgos, H.I., Pannunzio, V. et al. Brefeldin a decreases the activity of the general amino acid permease (GAP1) and the more specific systems for L-leucine uptake in Saccharomyces cerevisiae . Cell. Mol. Biol. Lett. 11, 256–263 (2006). https://doi.org/10.2478/s11658-006-0020-8

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  • DOI: https://doi.org/10.2478/s11658-006-0020-8

Key words

  • Brefeldin A
  • Leucine permeases
  • Saccharomyces cerevisiae

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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